Benchennouf et al. (2017) analysed the antioxidant activity of goji berries in their experiment of ripened fruits from organic farms. The methodology used a combination of chemicals and reagents including; Folin-Ciocalteu, gallic acid, TPTZ, EDTA, hydrogen peroxide, and luminol. The extraction process utilized Soxhlet apparatus together with appropriate solvents such as; hexane, dichloromethane, and methanol. While evaluating the antioxidant activity of the extracts, the DPPH method was used by adding 0.1mM methanol to different concentrations of the extract. The mixture was shaken vigorously and changes in the absorbance rates were read at 515nm at an interval of 30 minutes. The inhibition percentage was then calculated using an outlined formula. According to these procedures, the study by Benchennouf et al. (2017) as well as this project bear similarities to that of Wang et al. (2010) which also used the DPPH method. Furthermore, the inclusion of Folin-Ciocalteu reagents imply that Benchennouf et al. (2017) used procedures relating to the methodology of Bellaio, Carnevale, and Bona (2014).
Another experiment performed by Donno et al. (2015) was very comprehensive in determining the antioxidant activity of goji berries. In a similar approach as Benchennouf et al. (2017), the researchers collected sampled of goji berries from a local farm and used variety of chemicals and solvents. These included; sodium carbonate, TPTZ, EDTA, ethanol, ammonium dihydrogen phosphate, and cetyltrimethylammonium bromide. The test for antioxidant bioactivity was achieved through the actions of ferric reducing antioxidant power. Therefore, these procedures exhibit similarities with the methodology used by Wang et al. (2010) and Ruffo et al. (2017). On the contrary, the protocols do not properly fit the descriptions of this project which relied on the DPPH method and observations of the 96-well plates.