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experiment for chlorophyll and carotenoid determination

Inbaraj et al. (2008) conducted a similar experiment for chlorophyll and carotenoid determination. However, there was notable difference where the researcher did not use UV-vis spectrophotometer as integrated in this project. In addition, the experiment was dissimilar to the approach by Bertoldi et al. (2019). For instance, Inbaraj et al. (2008) did not use samples of goji berries from different regions rather the entire 1.8kg of fruits used was bought from local shops. Chemicals and reagents used by these researchers included; analytical grade solvent n-hexane, oleic acid methyl ester in the ratio 18:1, acetonitrile, and palmitic acid methyl ester in the ratio 16:0. In addition, Inbaraj et al. (2008) used unique apparatus different from those used in this project or even the experiment conducted by Bertoldi et al. (2019). The materials included; column oven controller, single quadrupole mass spectrometer, N-1 rotary evaporator, high performance liquid chromatography, and high-speed centrifuge. The process analysis of 1g of ground goji berries mixed with 20ml solution of hexane, ethanol, acetone, and toluene in the ratio of 10:6:7:7. From here, 1ml of 40% solution of methanolic potassium hydroxide was added and then placed in the dark for up to 12 hours. Later on, nitrogen was pumped in the mixture followed by 15ml of hexane and 15ml of anhydrous sodium sulfate solution (10%).

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