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experiment pertaining to the antioxidant scavenging assay in goji berries

Ruffo et al. (2017) also performed an experiment pertaining to the antioxidant scavenging assay in goji berries and the methodology revealed notable differences when compared to this project or Islam et al. (2017). That is, this methodology used control experiments in testing the antioxidant scavenging assay of two types of goji berries. Here, the tests were also conducted in three phases whereby the first one involved experimenting scavenging of effects of DPPH. The experiment started by adding 1ml of aqueous solution of goji extract to 4ml solution of ethanolic DPPH. The solutions were placed in a dark room for 15 minutes to record the inhibition percentage and the absorption rate. The second experiment involved ABTS radical scavenging assay whereby 7mM of aqueous ABTS solution was mixed with 2.45mM of potassium persulfate. After leaving the mixture in the dark for 16 hours, the absorption rate was measured at 734nm. The last experiment investigated the oxygen radical absorbance capacity (ORAC) assay by testing the 96-well plates as indicated in this project. Solutions of different concentrations were used in preparing the solution including; 75nM phosphate, 40 nM fluorescein, and 150nM AAPH. Therefore, despite the difference with Islam et al. (2017), the experiment of Ruffo et al. (2017) showed similarities with the methodology of this project.

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