The present study was conducted to establish a screening method of waterlogging tolerance in sesame at the germination stage and early seedling stage, and to identify the waterlogging tolerance varieties.
Materials and Methods
Plant materials
Fifty-eight (58) sesame accessions including 56 accessions of Sesamum indicum L. and 2 accessions of Sesamum radiatum Schum. & Thonn. were used in this study, which were collected from Myanmar (Supplemental Table 1). All the accessions were multiplied in a greenhouse at T-PIRC (Tsukuba Plant Innovation Research Center) of University of Tsukuba in 2018 crop season. Seeds were collected and stored at 4°C until the use in the experiment.
Methods of germination assay and stress treatment at seed stage (Laboratory screening)
The experiment was laid out in factorial arrangement with completely randomized design with three replications. Prior to the treatment, seed surface was sterilized with 70 % ethanol for about 5 minutes and rinsed with running water thrice. The seeds were submerged in 1ml tube with distilled water. The waterlogging treatments were established as a function of waterlogging days (3 days, 5 days, 7 days, 9 days and 15 days).) at ro