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Tests for Polyphenols
In this experiment, test for polyphenols used chemicals and reagents that are similar to the methodologies incorporated by Benchennouf et al. (2017) and Islam et al. (2017). On the contrary there were differences in terms of the procedures followed in this project as compared to those implemented by Benchennouf et al. (2017) and Islam et al. (2017). For instance, both experiments of Benchennouf et al. (2017) and Islam et al. (2017) used standard apparatus for Folin-Ciocalteu tests while it was observed that the project undertaken here prepared the polyphenolic reagents and FC assay. In their methodology, Benchennouf et al. (2017) used an assortment of reagents including sodium carbonate alongside Folin-Ciocalteu. The researchers used ground goji berries mixed with hexane, dichloromethane, and methanol in a bid to increase polarity of the extracts. Benchennouf et al. (2017) conducted their experiment by adding a mixture of 7900µl distilled water, 10µl diluted sample, and 50µl Folin-Ciocalteu reagent to an Eppendorf tube. Sodium carbonate (20%) was transferred to the vortex and left in dimly lit condition for 120 minutes. Thereafter, the researchers read absorbance of the solution at 70nm thereafter plotting a calibration curve in the range of 100 to 500µg/ml gallic equivalents.